Isolation and Identification of Mycoplasma and Detection of Drug Resistance Genes in Some Dairy Farms in Southern Xinjiang
In order to further investigate the molecular characteristics and drug resistance status of Mycoplasma in intensive dairy farms in Southern Xinjiang,nose swabs were collected from the calves with respiratory symptoms in 8 farms for isolation and identification of Mycoplasma as well as for drug resistance gene testing.The results revealed that the positive rate of Mycoplasma was 39.02％(16/41),involving 4 types,namely Mycoplasma bovis,Mycoplasma bovirhinis,Mycoplasma dispar and Mycoplasma arginini,all of which were 92.4％-100％identity to several epidemic strains abroad;after isolation and purification,a total of 4 strains of Mycoplasma bovis and 2 strains of Mycoplasma dispar were obtained.Free cell-wall and"fried egg like"colonies were observed under microscopic examination,and by Dienes staining,the colonies showed transparent circles with dark blue centers and light blue edges.Meanwhile,the biochemical identification results were consistent with the biochemical characteristics of Mycoplasma bovis and Mycoplasma dispar.All the detection rates of TEM,gyrA and tetC genes of the isolated strains of Mycoplasma bovis were 100％,that of parC was 75.0％,and all the detection rates of TEM,gyrA,parC and tetC genes of the isolated strains of Mycoplasma dispar were 100％,the proportions of isolates containing three or four drug resistant genes were 16.67％and 83.33％,respectively.Data support was provided for the prevention and control of bovine mycoplasmosis in the region,contributing to the guidance of clinical administration and development of vaccines of bovine mycoplasmosis.
Mycoplasma bovisMycoplasma disparisolation and identificationdrug resistance gene
万方数据
维普
