首页|荧光免疫层析技术原理及其在病原微生物检测中的研究进展

荧光免疫层析技术原理及其在病原微生物检测中的研究进展

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荧光免疫层析技术是近年来开发的一种新型免疫检测技术,以荧光微球为标记物,既保留了传统胶体金试纸条检测速度快、价格便宜、操作简单、携带方便等优点,又通过荧光技术提高了检测的灵敏度.荧光免疫层析技术对实验设备、资源的要求低,特别适合在条件有限的实验室及野外开展动物疾病快速诊断.本文详细介绍了荧光免疫层析技术的原理及其在病毒、细菌、寄生虫等病原检测方面的应用研究,以期为病原微生物现场快速诊断技术的开发提供参考.
The Principle of Fluorescence Immunochromatography and It Advances in Detection of Pathogenic Microorganisms
Fluorescence immunochromatography is a new immunodetection technology developed in recent years,which,taking fluorescent microsphere as a marker,not only retains the advantages of rapidity,low cost,simple operation and convenience,but also improves sensitivity with the help of fluorescence technology.The technology is particularly suitable for rapid diagnosis of animal diseases in laboratories with limited conditions and in the field,as no more experimental devices or resources are required.In the paper,the principle of the technology and its application in the detection of viruses,bacteria and parasites were introduced,with a view to providing a reference for on-site rapid detection of pathogenic microorganisms.

fluorescence immunochromatographyfluorescent microspheredetection of pathogenic microorganisms

苏华彬、刘蒙达、南文龙、孙明军、焉鑫、毛迎雪、曲瑶、孙淑芳、李桂梅、樊晓旭

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中国动物卫生与流行病学中心,山东青岛 266032

青岛农业大学动物医学院,山东青岛 266109

农业农村部反刍动物重大疫病防控重点实验室(东部),山东青岛 266032

山东农业大学动物科技学院,山东泰安 271000

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荧光免疫层析技术 荧光微球 病原微生物检测

"十四五"国家重点研发计划项目国家奶牛产业技术体系资助项目

2022YFD1800701CARS36

2024

中国动物检疫
中国动物卫生与流行病学中心

中国动物检疫

影响因子:0.437
ISSN:1005-944X
年,卷(期):2024.41(1)
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