On August 17,2021,clinical symptoms were found in a Simmental cow,including high fever,rumen flatulence,muscle tremor,tachypnea,conjunctival xanthochromia and hemoglobinuria,etc.In order to find out the cause of the disease,whole blood samples and tick samples on body surface of the cow were collected for optical microscopy observation,routine blood test(RBT)and polymerase chain reaction(PCR)assay.The results of blood smear microscopy showed that the cow was suspected to be infected with a mixture of pathogens including Theileria annulate,Candidatus midichloria sp.and Anaplasma ovis;RBT results showed that the indicators such as hemoglobin,hematocrit,average hemoglobin concentration and neutrophils decreased,but the platelet and ratio of lymphocyte increased,indicating that anemia and inflammatory reaction were available in the cow;PCR results showed that the blood samples were positive for Theileria annulate,Candidatus midichloria sp.and Anaplasma ovis;and tick samples were positive for Theileria annulate only.In conclusion,the infected cow was finally confirmed to be simultaneously infected with Theileria annulate,Candidatus midichloria sp.and Anaplasma ovis.Such co-infection with three pathogens had been seldomly found till now,future clinical diagnosis based on which was provided with a reference by the paper.
关键词
牛环形泰勒虫/念珠菌/绵羊无浆体/混合感染/进化树分析
Key words
Theileria annulata/Candidatus midichloria sp./Anaplasma ovis/co-infection/evolutionary tree analysis
维普
万方数据