Comparison of Three Methods for Detecting the Viral Load in Chicken-embryo Chicks Artificially Infected with REV
In order to compare the accuracy of various methods for detecting avian reticuloendotheliosis virus(REV)in blood and fecal samples and thus to provide a reference for the development of purification procedures for avian reticuloendotheliosis(RE),a model for REV vertical transmission to chicks was constructed using chickembryoyolksac inoculation.Blood and meconium samples from 1-day-old chicks,blood and cloacal swabs from 7 to 70-day-old chicks(every other week)were detected by RT-PCR,fluorescence quantitative RT-PCR and virus isolation method using DF-1 cells,respectively,and the results from which were compared.The results revealed that,for the plasma from 1-day-old SPF chicks,fluorescence quantitative RT-PCR had the highest sensitivity and positive detection rate of 100%,followed by virus isolation mehtod with the positive detection rate of 96%(24/25),and the last one was RT-PCR;for the meconium samples of 1-day-old SPF chicks,fluorescence quantitative RT-PCR and virus isolation shared the same sensitivity with the positive detection rate of 80%(20/25),and RT-PCR had the lowest detection rate of 28%(7/25).For the blood and cloacal swabs from 7 to 70-day-old chicks(every other week),the detection rates by the three methods were generally similar to those of 1-day-old chicks,that was,fluorescence quantitative RT-PCR,virus isolation and RT-PCR in successive order from high to low.In conclusion,the positive detection rate of REV in meconium/cloacal swabs was generally lower than that in blood using the same method for detecting samples within the same period.The methods for detecting the viral load of chicken-embryo chicks artificially infected with REV were preliminarily compared in the study,laying a foundation for future implementation of REV purification.
国家科技期刊平台
NSTL
万方数据
维普
