Molecular Tracing of a Positive Sample with Avian Influenza Virus by Nanopore Sequencing
H5 subtype avian influenza(AI)is a zoonotic disease posing a serious threat to human and animal health.It is important to disclose the molecular characteristics of avian influenza virus(AIV)through targeted whole genome sequencing and phylogenetic analysis.In the study,the whole genome of AIV in one positive swab sample was sequenced by the third-generation of targeted nanopore technology.The sequence identification results showed that H5N5 subtype AIV was identified,its sequence coverage was 96.44%,only leaving about 480 bp sequence of PB1 gene;HA protein cleavage site contained 5 consecutive alkaline amino acids,with the molecular characteristics of highly pathogenic avian influenza virus(HPAIV),and no amino acid mutation converting to SAα2-6 Gal receptor gene was found.HA gene phylogenetic analysis results showed that the virus fell into 2.3.4 lineage,and NA gene belonged to Eurasian lineage.The genome of AIV in the inactivated cloacal swab was successfully sequenced by targeted nanopore sequencing technology,verifying that the technology was rapid and convenient,could be directly used for whole genome sequencing of viruses in swab samples and for rapid molecular identification and traceability of animal influenza viruses.
国家科技期刊平台
NSTL
万方数据
维普
