山羊口蹄疫、小反刍兽疫疫苗不同免疫剂量同步分点注射免疫效果比较
Comparison on Immune Effects of Synchronous Multiple-point Injection with Goat FMD and PPR Vaccines at Different Doses
高丽艳 1杨晓燕 2台晓媛 1喻永福 1张俊雄 1李继明 1段纲 3李本能 4李学德 2陈关雄1
作者信息
- 1. 石林彝族自治县动物疫病预防控制中心,云南石林 652200
- 2. 云南鑫德顺畜牧有限公司,云南石林 652200
- 3. 云南农业大学动物医学院,云南昆明 650201
- 4. 会泽县待补镇农业农村综合服务中心,云南会泽 654216
- 折叠
摘要
为探究一种适合基层同时开展山羊口蹄疫(FMD)和小反刍兽疫(PPR)疫苗免疫的方法和剂量,选取64 只山羊随机分为 4组,即常规免疫组(对照组)以及 1.0、1.2和 1.5头份剂量组(试验组),开展FMD和PPR疫苗常规免疫和不同免疫剂量同步分点注射免疫的效果比较.结果显示:使用上述两种疫苗,采用常规免疫和一次性分点同步注射免疫的方法,以不同免疫剂量同步分点注射接种后,被免疫山羊未出现免疫应激反应;以不同免疫剂量接种后 14、28、42、56、70、100 和 160 d,各组山羊FMD O型、A型以及PPR免疫抗体阳性率差异均无统计学意义(P＞0.05).在接种后14 d,1.2头份剂量组FMD O型免疫抗体阳性率高于1.0和1.5头份剂量组;在接种后 100 d,1.5 头份剂量组FMD O型免疫抗体阳性率高于其余两剂量组;接种后 14 d,对照组山羊FMD A型免疫抗体阳性率为 87.5%,其余各组均可达到 100%,并维持至接种后 100 d.各组山羊PPR抗体阳性率均在接种后14 d达到100%,并持续保持至接种后160 d.结果表明:在试验免疫剂量范围内可开展山羊FMD和PPR疫苗一次性分点同步免疫接种工作,FMD疫苗最佳免疫注射剂量为 1.2 头份,PPR疫苗为 1.0头份,在接种 14 d后开展免疫效果监测较好.本研究为基层动物防疫员高效、便利开展山羊FMD和PPR疫苗免疫工作提供了参考.
Abstract
In order to explore a method and dosage for synchronous vaccination for goat foot-and-mouth disease(FMD)and peste des petits ruminants(PPR)at grassroots level,64 goats were randomly selected and divided into 4 groups:one group with routine vaccination(control group)and three experimental groups with synchronous multiple-point vaccination(groups of 1.0,1.2 and 1.5 doses),respectively,to compare the immune effects of different methods for FMD and PPR vaccines.The results showed that no immunological stress reaction was found in the vaccinated goats after the above injections with the two vaccines;and no statistically significant difference was detected among the positive rates of immune antibodies against FMD-O/A virus and PPR virus in each group for 14,28,42,56,70,100 and 160 days after vaccination at different doses(P＞0.05).On the 14th day after vaccination,the positive rate of antibodies against FMD-O virus in goats in the group of 1.2 doses was higher than that in the groups of 1.0 and 1.5 doses;on the 100th day after vaccination,the positive rate of antibodies against FMD-O virus in goats in the group of 1.5 doses was higher than that in other two experimental groups;on the 14th day after vaccination,the positive rate of antibodies against FMD-A virus in goats in control group was 87.5%;and that of other groups all reached up to 100%and lasted till on the 100th day.The positive rate of antibodies against PPR virus reached 100%in each group on the 14th day after vaccination and remained till on the 160th day.In conclusion,synchronous multiple-point vaccination with goat FMD and PPR vaccines could be conducted at the experimental doses,specifically,the doses of 1.2 and 1.0 were optimal for the vaccines of FMD and PPR,respectively,and better immune effect should be monitored on the 14th day after vaccination.Grassroots animal health workers were thus provided with a reference to efficiently and conveniently implement vaccination of FMD and PPR vaccines in goats.
关键词
口蹄疫/小反刍兽疫/免疫剂量/同步分点注射Key words
FMD/PPR/immune dose/synchronous multiple-point injection引用本文复制引用
基金项目
云南省创新与科技型企业培育计划项目(202104BI090017)
云南省重大科技专项计划项目(202302AA310020)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据