摘要
为探究不同佐剂对猪流行性腹泻灭活疫苗免疫效果的影响,选用水包油佐剂A(O/W)、油包水佐剂B(W/O)和双相佐剂C(W/O/W)3 种不同佐剂配制疫苗,分别接种 3～5 日龄仔猪后攻毒,综合评价不同佐剂的免疫效果.结果显示:A、B佐剂组疫苗免疫猪的抗体水平低于C佐剂组;攻毒后进行猪流行性腹泻病毒检测发现,仅C佐剂组试验猪全部呈阴性;剖检所有疫苗免疫组试验猪均未发现脏器病变.结果说明:双相佐剂制备的灭活疫苗免疫效果明显优于水包油和油包水佐剂疫苗,且安全性较高,可优先选择双相佐剂制备猪流行性腹泻灭活疫苗.本研究为猪流行性腹泻疫苗制备、生产提供了技术支撑.
Abstract
In order to investigate the effect of different adjuvants on inactivated vaccines against porcine epidemic diarrhea(PED),vaccines were prepared by three adjuvants,i.e.,oil-in-water adjuvant A(O/W),water-in-oil adjuvant B(W/O)and water-oil-water adjuvant C(W/O/W),to vaccinate piglets at the age of 3～5 days,respectively,followed by challenge test as well as comprehensive evaluation on the immunization effect of the adjuvants.The results showed that the antibody levels of the vaccinated pigs in A and B adjuvant groups were lower than those in C adjuvant group;it was detected that,after virus challenge,only all the piglets in C adjuvant group were negative;and no organ lesion was found in all vaccinated piglets under autopsy.In conclusion,inactivated vaccines prepared with water-oil-water adjuvants were obviously superior to those with water-in-oil or oil-in-water adjuvants,which was safer and could be prioritized to prepare inactivated vaccines against PED.The preparation and production of PED vaccines were technically supported by the study.
基金项目
陕西省教育厅服务地方专项(19JC043)
榆林学院高层次人才科研启动基金(20GK12)
榆林学院高层次人才科研启动基金(20GK13)
榆林市科技局产学研合作项目(2019-103-5)
国家科技期刊平台
NSTL
维普
万方数据