首页|2023年湖南省郴州市生猪养殖标准化示范场主要疫病抗体检测与分析

2023年湖南省郴州市生猪养殖标准化示范场主要疫病抗体检测与分析

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为了解湖南省郴州市生猪养殖标准化示范场猪群的血清学抗体水平,应用酶联免疫吸附试验(ELISA)对该市17个生猪养殖标准化示范场的431份猪血清样品进行了O型口蹄疫(FMD-O)、A型口蹄疫(FMD-A)、猪瘟(CSF)、猪繁殖与呼吸综合征(PRRS)、猪伪狂犬病(PR)等4种病种(6个指标)抗体检测,并使用R(V4.3.1)软件对地区分布、猪群规模、生长阶段等口蹄疫(FMD)免疫水平影响因素进行了卡方检验.结果显示:CSF、PRRS、FMD-A、FMD-O、PR-gB、PR-gE 的抗体阳性率分别为 86.54%、65.89%、79.81%、85.85%、85.38%、1.62%;郴州市西部猪场FMD免疫效果最好,北部最差,差异有统计学意义(P<0.05),不同养殖规模猪群间抗体阳性率没有统计学意义(P>0.05),不同生长阶段猪群间具有统计学意义(P<0.05),其中育肥猪最高.结果表明:2023年郴州市生猪养殖标准化示范场主要疫病免疫抗体水平较高,但部分地区猪场抗体个体阳性率偏低,且猪群中存在PR野毒感染,存在发病风险;地区分布和生长阶段是影响FMD免疫效果的重要因素.结果提示,应定期评估并调整相应的免疫或补免措施,做好动物疫病防控,确保猪群生产安全.
Detection and Analysis of Serological Antibodies against Major Animal Diseases on Standardized Demonstration Swine Farms in Chenzhou City,Hunan Province in 2023
In order to investigate the level of serological antibodies against major animal diseases on standardized demonstration swine farms in Chenzhou City,Hunan Province,431 swine serum samples collected from 17 farms were detected by enzyme-linked immunosorbent assay(ELISA)for antibodies against 4 diseases(involving 6 indicators),including foot-and-mouth disease type O(FMD-O),foot-and-mouth disease type A(FMD-A),classical swine fever(CSF),porcine reproductive and respiratory syndrome(PRRS)and pseudorabies(PR),and chi square test was conducted using R(V4.3.1)software for analysis of regional distribution,pig population size,growth stage and other factors influencing the immune level of FMD.The results showed that antibody positive rates of CSF,PRRS,FMD-A,FMD-O,PR-gB and PR-gE were 86.54%,65.89%,79.81%,85.85%,85.38%and 1.62%,respectively;for the immunization of FMD,the farms in western Chenzhou were the best,and those in northern Chenzhou were the worst,with a statistically significant difference(P<0.05);for antibody positive rate of pig populations at different growth stages,statistical significance was observed(P<0.05),especially the rate of fattening pigs was the highest,while that of pig populations with different sizes was not significant(P>0.05).In conclusion,the level of antibodies against major animal diseases was relatively high on standardized demonstration swine farms in Chenzhou City,Hunan Province in 2023,but the antibody positive rate at the individual level was lower in some regions.Meanwhile,wild PR virus was present in pig populations,posing a risk of outbreak;regional distribution and growth stage were crucial to the immune effect of FMD.Relevant vaccination or supplementary vaccination should be adjusted based on regular evaluation to better prevent and control animal diseases and safeguard the safety of pig production.

standardized demonstration swine farmpig diseaseserologyimmunization qualification rate

张智勇、陈南颖、陈文承

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郴州市动物疫病预防控制中心,湖南郴州 423000

标准化示范场 猪群疫病 血清学 免疫合格率

2024

中国动物检疫
中国动物卫生与流行病学中心

中国动物检疫

影响因子:0.437
ISSN:1005-944X
年,卷(期):2024.41(3)
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