摘要
为了解恒河猴在实验室饲养环境下的肠道寄生虫感染情况,采用直接涂片法和饱和食盐水漂浮法,对采集的66只试验用恒河猴新鲜粪便进行肠道寄生虫检查,并评估了血液学、血液生化学相关指标以及驱虫药物的驱虫效果.结果显示:从66只恒河猴新鲜粪便中检出类圆线虫和鞭虫2种肠道蠕虫卵囊,感染率分别是9.1%和 12.1%,均为单一感染且感染强度较低;感染类圆线虫或鞭虫恒河猴的血液学指标(WBC、RBC、HGB、MCH、MCHC、HCT、MCV、PLT、PDW、MPV、PCT、LYMPH、MONO、EO、BASO)和肝肾功能指标(ALT、AST、ALP、LDH、TP、BUN、CREA)与健康动物无显著差异(P＞0.05);连续使用伊维菌素注射液或阿苯达唑伊维菌素片均能有效驱除感染的类圆线虫或鞭虫.结果说明:恒河猴在实验室饲养环境下,其肠道寄生虫的感染种类较少,感染率和感染强度均较低,对动物健康状况无明显影响,通过良好的饲养管理和完善的药物驱虫程序能实现对猴肠道寄生虫病的有效控制.
Abstract
In order to investigate the infection with intestinal parasites in rhesus monkeys under laboratory environment,fresh feces from 66 experimental rhesus monkeys were detected for intestinal parasites using direct smear and saturated saline floatation methods,haematological and haematobiochemical indicators as well as anthelmintic efficacy of anthelmintic drugs were evaluated.The results showed that two types of intestinal worm oocysts including Strongyloides and Trichuridae were detected out,with infection rates of 9.1%and 12.1%,respectively,both of which were single infection with low intensify;for the infected monkeys,the haematological indicators(WBC,RBC,HGB,MCH,MCHC,HCT,MCV,PLT,PDW,MPV,PCT,LYMPH,MONO,EO and BASO)and hepatorenal function indicators(ALT,AST,ALP,LDH,TP,BUN and CREA)were not significantly different from those of healthy animals(P＞0.05);and the active Strongyloides or Trichuridae could be effectively removed by continuously using ivermectin injection or albendazole ivermectin tablets.In conclusion,the rhesus monkeys were infected with fewer types of intestinal parasites under experimental environment,with low infection rate and intensity,imposing little effect on animal health,and could effectively recover through good feeding management and sound procedure of drug deworming.
基金项目
广东省重点领域研发计划(2023B1111050008)
广东省重大人才工程项目(210183503006)
广东省实验室建设项目(2023B1212070029)
国家科技期刊平台
NSTL
维普
万方数据