Efficiency Enhancement of Immune Response to Avian Influenza-Newcastle Disease Vaccine by Carbon Nanotube
In order to investigate the efficiency of immune response to avian influenza-Newcastle disease bicombinant inactivated vaccine by carbon nanotube(CNT),chickens were immunized with the prepared CNT-bicombinant vaccine mixture,from which the antibodies produced were continuously detected using haemagglutination inhibition(HI)test,and the average antibody titer and antibody positive rate were statistically analyzed.The antibody titer detection results showed that,7 days after immunization,the antibody titers of Newcastle disease and avian influenza in the high-dose CNT group were both higher than those in the control group;14-130 days after immunization,the antibody titers in the high-dose CNT group were stable at above 8.00 Log2;7-31 days after immunization,the average antibody titers in the medium and low-dose CNT groups gradually increased,with not significant difference compared to the control group;42-130 days after immunization,the average antibody titers in the medium and low-dose groups gradually decreased,but that in the control group was more obvious.The antibody positive rate results showed that the antibody positive rates of Newcastle disease and avian influenza in medium-dose control groups started to decrease after 42 and 70 days,respectively,while those in the CNT group remained 100%;at the late stage of monitoring period(90～130 days after immunization),the rate in the low-dose CNT group were significantly higher than that in the control group,with statistically significant difference(P＜0.05).In conclusion,CNT,as an immune enhancer,was effective to accelerate immune response,maintain the level of antibody titer and reduce the dosage of immunogen.A new idea was thus provided for the development and application of immune enhancers in animals.
国家科技期刊平台
NSTL
万方数据
维普
