Research Progress on Serological Detection Methods for Lumpy Skin Disease
Lumpy skin disease(LSD)has been posing a threat to China's cattle industry,which could be detected by serological methods that were convenient,sensitive and economic,and early detection and evaluation on vaccination effect were also crucial for disease control.In the paper,research progress on various common serological methods for detection of LSD was introduced,the advantages and disadvantages of each method as well as its applicable scenarios were analyzed.Viral neutralization test(VNT),as the gold standard for detecting antibodies against LSD virus,could not be used in the grassroots laboratories due to its long test period and strict operational requirements,but was more suitable for confirming suspected cases;Enzyme-linked immunosorbent assay(ELISA)was appropriate for detection of large number of samples due to its accessibility and short reaction time,but the immune mechanism of antigens was less developed with immature methods;Indirect immunofluorescent antibody test(IFAT)was difficult for clinical detection due to its low specificity in spite of high sensitivity;Immunoperoxidase monolayer assay(IPMA)could be used to detect certain samples,but the results were subject to single-hole microscopy,which was not as clear as those by ELISA;Western blotting(WB)could identify parapoxviruses,but couldn't be used in detection large quantities of samples due to its cumbersome operation.In a word,ELISA was superior in clinical serological detection of LSD.Basic researches on LSD virus should be improved in the future to clarify the immune mechanism of each protein,and to screen out effective and sensitive antigens,so as to establish a method for detection of large quantities of samples at the grassroots level.
国家科技期刊平台
NSTL
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