Development of Fluorescence Microsphere Immunochromatographic Strips for Brucellosis
In this study,immunochromatographic strips were prepared taking quantum dot fluorescent microspheres as markers for rapid detection of bovine serum antibodies against Brucella.Brucella lipopolysaccharide(LPS)and rabbit polyclonal antibodies against recombinant streptococcal protein G were sprayed onto nitrocellulose membranes as detection and quality control lines,respectively.Recombinant streptococcal protein G labeled with fluorescent microspheres were sprayed onto glass cellulose membranes as quantum dot binding pads.After assembly and cutting,the immunochromatographic strips were prepared.The sensitivity and stability of the prepared strips were determined using national standard brucellosis positive serum,and the specificity was determined using common bovine disease serum including bovine rhinotracheitis positive serum and national standard brucellosis negative serum.100 clinical serum samples were synchronously tested using the prepared strips and indirect ELISA(iELISA)to compare the compliance rate of the two methods.The results showed that the lowest detection limit of the prepared strips was 0.3 IU/mL,and the sensitivity remained unchanged after storage for 18 months under room temperature;no cross reaction with negative serum of brucellosis or positive serum of bovine rhinotracheitis,bovine epidemic diarrhea and lumpy skin disease was found;compared with iELISA,the positive,negative and overall compliance rates were 94.2%,100%and 97.0%,respectively.In conclusion,the prepared strips were sensitive,specific and stable for rapid clinical detection without aid of instruments,supporting the prevention and control of brucellosis in China.
国家科技期刊平台
NSTL
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