Preparation and Comparison of Colloidal Gold Immunochromatographic Strips for Canine Brucellosis Based on Different Antigens
In order to develop a colloidal gold immunochromatographic method for rapid detection of canine brucellosis,strips were prepared respectively using membrane proteins and soluble proteins extracted from canine B.canis,outer membrane proteins of Omp31 and BP26 purified by prokaryotic expression system,as well as fusion protein of F14 based on B cell antigen peptides.The sensitivity and specificity of the strips were compared respectively using canine brucellosis-positive and-negative sera.The results showed that for sensitivity,Omp31 antigen was the highest,followed by F14 and BP26,but membrane proteins and soluble proteins were poor;for specificity,30 canine brucellosis negative sera were tested by the colloidal gold strips prepared by five antigens,with negative results;there was no cross reaction to the positive sera of Salmonella,Escherichia coli(O157),Escherichia coli(H7),Vibrio cholerae,Vibrio parahaemolyticus and Legionella.In addition,the strips prepared based on F14 and BP26 protein could be used to recognize positive serum of sheep brucellosis,while those prepared based on Omp31 protein,cell membrane protein and soluble protein failed for positive sera of both cattle and sheep brucellosis.In conclusion,Omp31,F14 and BP26 protein could be used as candidates to prepare immunochromatographic strips for canine brucellosis.The diagnosis of brucellosis caused by rough Brucella could be technically supported by the study.
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