Investigation and Genotype Identification of Sheep Echinococcus in Yanggu County,Shandong Province
In order to investigate the prevalence of sheep echinococcosis in Yanggu County,Shandong Province,1 332 viscera of slaughtered sheep collected from 4 townships in the county from March to June 2023 were clinically examined,920 serum samples from sheep farms were tested for IgG antibodies using ELISA,and the mitochondrial cox1 genes of 5 Echinococcus-positive samples were amplified,sequenced and analyzed.The results showed that Echinococcus was detected in the livers or lungs of 18 sheep,with an average positive rate of 1.35%(18/1 322);17 serum samples were positive for IgG antibodies of Echinococcus,with an average positive rate of 1.85%(17/920),the infection rate was statistically different in different townships(P＜0.05),and the antibody positive rate of sheep≥6 years old was obviously higher than that of those＜6 years old(P＜0.05);the infection rate on intensive farms was significantly higher than that on free-range farms(P＜0.05),while the rate was unrelated to the species and sex;the 5 positive samples were the highest identical to G1 genotype of Echinococcus granulosus strain.In conclusion,sheep echinococcosis was available in Yanggu County,with the infection rate mostly affected by the feeding mode and age rather than the species and sex,and G1 genotype was dominant,posing a risk of zoonotic infection.It was proposed to improve researches related to molecular epidemiologic investigation on Echinococcus and operators'awareness about the disease.
echinococcosisinfection statusgenotype identificationinvestigation and analysis
国家科技期刊平台
NSTL
万方数据
