一株野鸭源禽副黏病毒6型的基因组特征分析
Genome Characterization of Avian Paramyxovirus Type 6 Derived from Wild Ducks
丁雨 1李旭勇 2陈璐 3张昕悦 4于晓慧 5周婉婷 6王静静 7刘华雷5
作者信息
- 1. 宁夏大学,宁夏银川 750000;中国动物卫生与流行病学中心,山东青岛 266032
- 2. 聊城大学,山东聊城 252000
- 3. 中国动物卫生与流行病学中心,山东青岛 266032;山东农业大学,山东泰安 271000
- 4. 中国动物卫生与流行病学中心,山东青岛 266032;河北工程大学,河北邯郸 056000
- 5. 中国动物卫生与流行病学中心,山东青岛 266032
- 6. 中国动物卫生与流行病学中心,山东青岛 266032;浙江农林大学,浙江杭州 310000
- 7. 中国动物卫生与流行病学中心,山东青岛 266032;农业农村部动物生物安全风险预警及防控重点实验室(南方),山东青岛 266032
- 折叠
摘要
为了解我国野生水禽中禽副黏病毒 6 型(APMV-6)的生物学特性,对 2020 年分离的 1 株野鸭源APMV-6(Wild duck/Shandong/W683/2020)进行了基因组测序及序列分析.结果显示:该APMV-6 分离株基因组为单股负链RNA,基因组长度为 16 230 nt,基因组结构为 3'-N-P-M-F-SH-HN-L-5',3'前导序列和 5'尾随序列长度分别为 55、54 nt,各基因间隔序列长度不等(2～63 nt);与APMV-1 疫苗株La Sota的R值为 0.167,抗原性存在较大差异;F蛋白裂解位点为 104IREPR↓L109,具有典型低致病性APMV分子特征.同源性分析发现,与台湾分离株(MZ802803)同源性最高(96.0%),与其他血清型APMV同源性仅为 63.1%～70.2%,与我国雁形目APMV-6 分离株(MZ802803)位于同一分支.本研究为进一步了解我国野鸟源APMV-6 基因组和生物学特性奠定了基础.
Abstract
In order to identify the biological characteristics of avian paramyxovirus type 6(APMV-6)present in wild waterfowl in China,one APMV-6 strain(wild duck/Shandong/W683/2020)isolated in 2020 was sequenced and analyzed.The results showed that the genome of the isolate was a single negative-strand RNA with the genome length of 16 230 nt and the structure of 3'-N-P-M-F-SH-HN-L-5',the length of 3'leader sequence and 5'trailing sequence was 55 and 54 nt,respectively,and the length of each gene interval sequence varied from 2 to 63 nt;the R value between it with vaccine strain La Sota was 0.167,and the antigenicity was greatly different;the cleavage site of F protein was 104IREPR↓L109,showing the typical low pathogenic APMV molecular characteristic.Homology analysis showed that it was the highest identical(96.0%)to Taiwan strain(MZ802803),but 63.1%～70.2%to other serotypes of APMV,locating in the same branch as the APMV-6 strain isolated from antheriformes in China(MZ802803).A basis was laid for further understanding of the genome and biological characteristics of APMV-6 derived from wild birds in China.
关键词
禽副黏病毒6型/野鸭/基因组序列Key words
APMV-6/wild duck/genome sequence引用本文复制引用
基金项目
国家重点研发计划项目(2022YFD1800600)
出版年
2024
国家科技期刊平台
NSTL
万方数据