Advances in Detection Methods for Swine Acute Diarrhea Syndrome
Swine acute diarrhea syndrome virus(SADS-CoV),as a novel porcine coronavirus originating from chrysanthemum bats,was firstly found from diarrhea piglets in Guangdong Province of China at the beginning of 2017,and caused two outbreaks in the province,not only posing a challenge to pig industry,but also leading to potential risks of public health.Considering the lack of effective drugs or vaccines,in order to implement efficient surveillance to control any prevalence of SADS-CoV and to help relevant departments to select appropriate methods,the research progress on detection methods for SADS-CoV were reviewed,including virus isolation and identification,RT-PCR,nested RT-PCR,real-time fluorescence quantitative PCR(qPCR),reverse transcription loop-mediated isothermal amplification(RT-LAMP),droplet digital polymerase chain reaction(ddPCR),reverse transcription polymerase amplification(RT-RPA),genome sequencing technology(NGS),immunofluorescence assay(IFA),luciferase immunoprecipitation system(LIPS),immunohistochemistry(IHC)and enzyme-linked immunosorbent assay(ELISA).It was analyzed that,any single method usually failed for current clinical testing due to its unique advantages and disadvantages as well as applicable scenarios,which should be used together with other methods to rapidly and accurately detect viruses by complementing each other's advantages that would make the detection more simple,efficient and cost-benefit.Future researches would focus on the methods with the functions of simple operation of virus detection,rapid and simultaneous detection of various viruses.It was believed that,with continuous improvement and innovation of detection technologies,more new methods would be used for SADS-CoV detection.
SADS-CoVisolation and identificationmolecular biological testingserological testing
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