Development of a Universal RT-RAA-LFD Method for Rapid Detection of FMDV
In order to develop a universal method to rapidly and effectively detect the foot and mouth disease virus(FMDV),a new method of reverse transcription recombinase-aided amplification combined with lateral flow dipstick(RT-RAA-LFD)was developed by designing labeling primers and probes targeting at FMDV 3D gene.The established method was efficient and convenient,which could complete detection within 35 min at 40℃and determine results through visual observation;with good coverage,major prevalent topological viruses including FMDV O and A type in China could be detected,and no cross-reaction with other swine pathogens such as classical swine fever virus(CSFV)and porcine pseudorabies virus(PRV)was observed;and the lowest detection limit to RNA standard substances was 10-7 copies/μL,with high sensitivity.5 clinical samples were detected by the established FMDV RT-RAA-LFD method,receiving consistent results with those by RT-PCR.In conclusion,the established method,with the advantages of rapidness,visualization,strong specificity and good repeatability,was appropriate for grass-roots animal disease prevention departments and farms.
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