3种非洲猪瘟病毒血清学检测试剂盒的比对
Comparison on 3 Kinds of Serological Detection Kits for African Swine Fever Virus
冯桂丹 1陶田谷晟 1向蒙 1杨显超 1王建 1赵洪进1
作者信息
- 1. 上海市动物疫病预防控制中心,上海 201103
- 折叠
摘要
为评估不同非洲猪瘟病毒(ASFV)检测试剂的检测性能,采用标准阳性血清、标准阴性血清和临床血清,比较了酶联免疫吸附试验(ELISA)、化学发光免疫分析(CLIA)、高敏荧光免疫分析 3 种ASFV血清抗体检测方法试剂盒的敏感性、特异性、符合率及临床应用效果.分析发现:4 个ELISA试剂盒、1 个CLIA试剂盒和 1 个高敏荧光免疫分析试剂盒在检测标准血清时,其敏感性、特异性和符合率差异不显著.3 种试剂盒的检测临界值均可达到 16 倍稀释,但在 32 倍以上稀释时各试剂盒有所差异,ELISA试剂盒的检测灵敏度总体较高.用历年不同非洲猪瘟疫情猪场的临床血清进行检测,发现不同试剂盒检测的阳性率存在差异,ELISA试剂盒的阳性检出率整体优于其他 2 种方法的试剂盒.综上表明,3 种方法的试剂盒在ASFV检测中都有一定的应用价值,其中ELISA试剂盒具有一定的优势,适用于ASFV的大规模筛查与监测.
Abstract
In order to compare the performance of different kinds of kits for detection of African swine fever virus(ASFV),the sensitivity,specificity,compliance rate and clinical application of 3 serological detection kits including enzyme-linked immunosorbent assay(ELISA),chemiluminescent immunoassay(CLIA)and high-sensitivity fluorescent immunoassay(HSIA)were compared using standard-positive sera,standard-negative sera and clinical sera.The results showed that the difference in the sensitivity,specificity and coincidence rate of 4 ELISA kits,1 CLIA kit and 1 HSIA kit was not significant.The detection critical value of the 3 kinds of kits could all reach 16 times dilution,but different when the dilution was above 32 times,and ELISA kit was generally more sensitive.When clinical sera collected from different farms with outbreaks of ASF in the past years were detected,it was found that the positive rates of different kits were different,and better in ELISA kit compared to the others.In conclusion,all of kits could be used to detect ASFV,especially ELISA kit that was superior in mass screening and monitoring of ASFV.
关键词
非洲猪瘟/酶联免疫吸附试验/化学发光免疫分析/高敏免疫分析Key words
ASF/ELISA/CLIA/HSIA引用本文复制引用
基金项目
上海市科技兴农项目(沪农科推字2021第1-6号)
出版年
2024
国家科技期刊平台
NSTL
万方数据