Evaluation on the Disinfection Effects of 5 Disinfectants Commonly Used by Swine Farms against PCV2 and PRV
In order to evaluate the safety of 5 disinfectants containing iodine,quaternary ammonium salts,potassium bisulfate,chlorine dioxide or glutaraldehyde commonly used by swine farms and their disinfection effects on viruses,their working concentrations were designed as high,medium and low under laboratory and simulated farm conditions according to the dilution ratios recommended by their instructions.After the disinfectants reacting with PCV2 and PRV for different time,the reaction product was inoculated into cells,and cell lesions were observed,the viral inactivation rate was calculated by TCID50,and the viral nucleic acid content was detected by fluorescence quantitative PCR.The results showed that,the disinfectants containing iodine and chlorine dioxide were less toxic to the cells,but those containing glutaraldehyde was on the contrary.All the 5 disinfectants could kill PCV2 and PRV to certain extent,the lower the concentration was,the longer the inactivation period became;under laboratory conditions,the disinfectants containing iodine,potassium bisulfate or chlorine dioxide,with stronger disinfection,could inactivate 100%viruses within 10 minutes at high and medium concentrations,while under simulated farm conditions,the disinfectants containing iodine were poor in disinfection,and those containing quaternary ammonium salts were poor in the removal of nucleic acids.In conclusion,all the 5 disinfectants could be used for disinfection on swine farms according to actual conditions and requirements;higher concentrations and enough disinfection time should be ensured as much as possible under the premise of ensuring safety.In the study,the correlation of inactivation effect of 5 disinfectants and the working concentrations and disinfection time were determined,providing a reference for the selection and use of disinfectants on swine farms.
国家科技期刊平台
NSTL
万方数据
