Whole Genome Sequencing Analysis on Genomic Characteristics and Antimicrobial Resistance of Avian Campylobacter in Jiaodong Region,Shandong Province
In order to identify the drug resistance,virulence and genotype genetic evolution of avian Campylobacter in Jiaodong region of Shandong Province,31 strains of Campylobacter isolated from 26 poultry farms in the region from 2021 to 2022 were tested for antibiotic susceptibility using broth dilution method,and their resistance genes,virulence genes,and multilocus sequence typing were analyzed based on the results of whole genome sequencing(WGS),followed by genetic evolutionary analysis on whole genome single nucleotide polymorphisms(wgSNPs).The results showed that the 96.77%of 31 strains were resistant to tetracycline,93.55%resistant to ciprofloxacin and nalidixic acid;for the 31 strains of Campylobacter,6 resistance spectrums were obtained and 15 strains were resistant to 3 or more drugs.WGS results showed that the 31 strains could be classified into 20 sequence types(STs),specifically,the dominant clonal group of Campylobacter colonis(C.colonis)was CC828,while Campylobacter jejuni(C.jejuni)showed diversity;antibiotic resistance genes associated with quinolones,tetracycline,aminoglycosides and β-lactam were found,42 virulence genes of 6 classes with known functions were carried.The wgSNPs phylogenetic analysis indicated that the dominant clonal group CC828 was distributed in 3 sub-clades,and C.jejuni was dispersedly in 7 sub-clades.In conclusion,the antimicrobial resistance of avian Campylobacter was relatively severe in Jiaodong region,with a wide range of drug resistance spectrums,and the drug-resistant genes carried were related to the drug-resistant phenotypes to certain extent;a large amount of virulence genes were carried,a dominant clonal group of C.colonis was found,ST-type of C.jejuni was various,and the isolated strains were genetically diverse.A technical support was provided for reducing the probability of transmission of avian Campylobacter to humans under the concept of"One Health".
万方数据
维普
