Antibacterial Effect of Asystasiella neesiana(Wall.)Lindau Alcohol Extract on Antibiotic-resistant Bovine Klebsiella pneumoniae in Vitro
In order to evaluate the antibacterial effect of Asystasiella neesiana(Wall.)Lindau alcohol extract on bovine Klebsiella pneumoniae,antibiotic resistance of Klebsiella pneumoniae strains isolated from cattle farms in Southeast Guizhou Province was analyzed by Kirby-Bauer method,the diameters of the inhibition circles of Asystasiella neesiana(Wall.)Lindau alcohol extract on the strains were measured by agar diffusion method,and then the minimum inhibitory concentration(MIC)and the minimum bactericidal concentration(MBC)of the alcohol extract were determined by the microdilution method and colony counting method.The results showed that the strains were insensitive to gentamicin,ampicillin,and trimethoprim/sulfamethoxazole,but sensitive to ciprofloxacin and ceftriaxone;the alcohol extract exhibited an inhibition diameter of(30.54±0.55)mm against the antibiotic-resistant Klebsiella pneumoniae strains,and was superior to ceftriaxone;the MIC and MBC were 0.78 mg/mL and 1.56 mg/mL,respectively.In conclusion,the Asystasiella neesiana(Wall.)Lindau alcohol extract had good antibacterial effect on the multi-drug resistance Klebsiella pneumoniae in vitro,indicating a potential prospect in livestock industry where antibiotic resistance was seriously and widely distributed.
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