Bioinformatics Analysis of pknB Gene of Mycobacterium tuberculosis var.bovis
In order to verify the biological functions of key protein of Mycobacterium tuberculosis var.bovis(MTB),the structure and biological properties of serine/threonine protein kinase pknB protein were predicted using the bioinformatics method.The results showed that pknB protein belonged to type Ⅱ transmembrane protein with a transmembrane region located in the middle of the protein,which was strongly hydrophilic without a signal peptide for secretion expression;random coils and α-helixes accounted for high proportions in the high-level structure,which were 44.73%and 32.27%,respectively,little extension strands and β-turns were contained;pknB protein was most likely located in the endoplasmic reticulum of cells,then in cytoplasm,but least likely in nucleus,golgi apparatus and mitochondria;many dominant antigenic epitopes were available,including 12 B-cell antigenic epitopes and 8 T-cell epitopes with above 25 scores;there were 65 potential phosphorylation modification sites that might interact with a variety of proteins,such as MRA_3902,MRA_3400,cfp17,etc.In conclusion,as a transmembrane protein,pknB was with good immunogenicity and could affect bacterial proliferation as an actor in the NF-κB signaling pathway of hosts,hence could be used as a candidate target protein for the development of vaccines and rapid diagnostic kits of tuberculosis.
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