Advances in Molecular Typing Techniques for Salmonella
Compared with traditional typing techniques,molecular typing techniques,with the advantages of high resolution,high repeatability and standardization,have been used as the key tool in the research fields of Salmonella pathogenicity,genetic association,traceability and transmission pathways.Molecular typing techniques for Salmonella are mainly classified as:restriction enzyme-based techniques centering on pulsed field gel electrophoresis(PFGE)and amplified fragment length polymorphism(AFLP),PCR amplification-based techniques centering on enterobacterial repetitive intergenic consensus PCR(ERIC-PCR)and multiple locus VNTRs analysis(MLVA),and sequencing-based techniques centering on multilocus sequence typing(MLST),clustered regularly interspaced short palindromic repeats(CRISP)and whole genome sequencing(WGS).All the three categories of techniques are characterized by the advantages of high sensitivity,good repeatability and accurate results,which helps to reveal the transmission relationship of hosts and regions among different Salmonella.For the purpose of accurate typing of Salmonella,one or several of these techniques could be used or combined according to laboratory conditions and research needs in practice due to their respective limitations to different extents.Here,domestic and foreign research progress on the three categories of techniques was summarized,aiming to provide a reference for the monitoring,risk assessment,traceability and early warning of Salmonella in food.
国家科技期刊平台
NSTL
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