Comparison of Combinations of Fluorescent Quantitative PCR Primers and Probe for Bovine Tuberculosis
In order to compare the specificity and sensitivity of different combinations of fluorescent quantitative PCR(qPCR)primers and probe for bovine tuberculosis,and to verify their clinical effects,11 combinations proposed by international,national,industrial and local standards and reported by literatures were compared and verified using national secondary standard materials of Mycobacterium bovis genomic DNA,nucleic acids of reference strain as well as clinical milk samples.The results showed that the 11 combinations all had no non-specific amplification,indicating good specificity;the primers originated from national standard IS1081 could reach a minimum detection limit of 1.4×10-1 with the highest sensitivity;the reproducibility of other combinations was good(CV＜10%),except for the national standard primers(Mtb);for 30 clinical samples,the detection rate of the positive samples detected by the primers originated from the national standard IS1081 was significantly higher than that by other combinations.In conclusion,the combination of primers and probe in national standard IS1081 was specific and sensitive,which could receive better clinical effects and was proposed to detect bovine tuberculosis by qPCR in laboratory.
bovine tuberculosisMycobacterium tuberculosis complexMycobacterium bovisfluorescent PCRprimer and probe
国家科技期刊平台
NSTL
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