近年来我国西部地区小反刍兽疫病毒N、F基因的遗传演化分析
Genetic Evolution Analysis on N and F Genes of Peste des petits Ruminants Virus in Western China
徐蛟 1苏娜 2王英丽 1刘珊 1张永强 1邹艳丽 1任炜杰 1包静月 1王志亮1
作者信息
- 1. 中国动物卫生与流行病学中心,山东青岛 266032
- 2. 青岛农业大学,山东青岛 266109
- 折叠
摘要
为了解近年来我国西部地区小反刍兽疫病毒(PPRV)的遗传演化情况,收集 2020-2024 年新疆、西藏、青海部分地区报告的 5 起小反刍兽疫(PPR)疫情发病动物的组织和拭子样品,利用荧光RT-PCR对PPRV进行初步检测,并对PPRV的N和F基因进行同源性比对和系统进化树绘制.结果显示:本研究检出的PPRV阳性样品均属于谱系IV型;与2014年以来国内的PPRV流行毒株以及蒙古国野生动物PPRV毒株亲缘关系较近,与除蒙古国外的其他国家流行株同源性较低;从野生岩羊和家羊中检出的PPRV阳性样品在进化树上没有显著差别.结果说明:2020-2024年我国西部地区发生的5起PPR疫情可能均与国内的流行株相关,病毒从周边国家传入的可能性较小,病毒暂未发生明显变化,家羊与野羊感染毒株具有相似的遗传来源.
Abstract
In order to investigate the genetic evolution of peste des petits ruminants virus(PPRV)in western China in recent years,tissue and swab samples were collected from infected animals in 5 outbreaks of peste des petits ruminant(PPR)reported in Xinjiang Uygur Autonomous Region,Xizang Autonomous Region,Qinghai Province from 2020 to 2024 to detect PPRV by fluorescent RT-PCR,followed by homology comparison of N and F genes of the virus and construction of a phylogenetic tree.The results showed that all the positive samples belonged to lineage IV,close to the PPRV strain in China since 2014 and that derived from wildlife in Mongolia,and less homologous to the strains in other countries except Mongolia;no significant difference was observed among the positive samples from wild blue sheep and domestic sheep in genetic evolution according to the evolutionary tree.In conclusion,the 5 PPR outbreaks in western China from 2020 to 2024 might be associated with domestic strains,with little probability of introduction from neighbouring countries,and the virus had not changed significantly,the strains isolated from domestic and wild sheep were with similar genetic origin.
关键词
小反刍兽疫/疫情/诊断/遗传演化Key words
PPR/outbreak/diagnosis/genetic evolution引用本文复制引用
基金项目
"十四五"国家重点研发计划项目(2022YFD1800500)
出版年
2024
国家科技期刊平台
NSTL
万方数据