Genetic Evolution Analysis on N and F Genes of Peste des petits Ruminants Virus in Western China
In order to investigate the genetic evolution of peste des petits ruminants virus(PPRV)in western China in recent years,tissue and swab samples were collected from infected animals in 5 outbreaks of peste des petits ruminant(PPR)reported in Xinjiang Uygur Autonomous Region,Xizang Autonomous Region,Qinghai Province from 2020 to 2024 to detect PPRV by fluorescent RT-PCR,followed by homology comparison of N and F genes of the virus and construction of a phylogenetic tree.The results showed that all the positive samples belonged to lineage IV,close to the PPRV strain in China since 2014 and that derived from wildlife in Mongolia,and less homologous to the strains in other countries except Mongolia;no significant difference was observed among the positive samples from wild blue sheep and domestic sheep in genetic evolution according to the evolutionary tree.In conclusion,the 5 PPR outbreaks in western China from 2020 to 2024 might be associated with domestic strains,with little probability of introduction from neighbouring countries,and the virus had not changed significantly,the strains isolated from domestic and wild sheep were with similar genetic origin.
国家科技期刊平台
NSTL
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