摘要
为了解四川省规模牛场牛结核病流行情况,采用牛结核分枝杆菌IFN-γ抗体夹心ELISA检测方法,对30 个牛场采集的 910 份牛全血样品进行了检测,并运用卡方检验对不同品种、不同养殖规模及不同海拔高度样品的检测结果进行了统计分析.结果显示:910份样品的个体表观阳性率为9.34%,个体真实阳性率为6.40%,场群阳性率为46.67%;奶牛场和肉牛场的个体表观阳性率分别为12.00%和8.82%,无统计学差异(P＞0.05);小型规模场和大型规模场的个体表观阳性率分别为10.00%和5.00%,无统计学差异(P＞0.05);低海拔牛场和高海拔牛场的个体表观阳性率分别为10.53%和3.33%,差异显著(P＜0.05).结果说明:四川省规模牛场牛结核病流行水平较高,尤其是低海拔牛场流行严重,而品种和养殖规模对牛结核病流行无显著影响.建议持续进行牛结核病风险监测,重点加强低海拔地区牛场的饲养管理,同时提高养殖场户的主体防疫意识,从源头阻断牛结核病传播.
Abstract
In order to investigate the prevalence of bovine tuberculosis on intensive farms in Sichuan Province,910 bovine whole blood samples collected from 30 farms were tested by Mycobacterium bovis IFN-γ ELISA kit,and the results of the samples from different species,breeding sizes and altitudes were analyzed using chi-square test.The results showed that individual apparent positive rate,individual true positive rate and farm/herd positive rate were 9.34%,6.40%and 46.67%,respectively;the individual apparent positive rates were 12.00%and 8.82%on dairy cattle farms and beef cattle farms,respectively,without statistical difference(P＞0.05),and the rates were 10.00%and 5.00%on small intensive farms and large ones,respectively,also without statistical difference(P＞0.05),while 10.53%and 3.33%on the farms at low altitude and those at high altitude,respectively,with significant difference(P＜0.05).In conclusion,bovine tuberculosis was prevalent at a high level on intensive farms in Sichuan Province,especially on those at low altitude,but the prevalence was not affected by cattle species and breeding sizes.It was recommended to continuously monitor possible risk of bovine tuberculosis,to strengthen feeding management on the farms at low altitude,and to improve farmers'awareness of disease prevention as key actors,so as to block possible transmission of the disease from its source.
维普
万方数据