Detection of Drug Resistance and Virulence Genes of Staphylococcus aureus in Dairy Cattle in Shandong Province
Staphylococcus aureus(S.aureus)with the capacity of drug resistance is emerging under global crisis of antibiotic resistance,posing a serious threat to public health.In order to investigate the prevalence of S.aureus and the carriage of virulence genes in dairy cattle farms in Shandong Province,from 2022 to 2023,520 milk samples were collected from cattle infected with mastitis in Jinan,Dezhou,Tai'an and Linyi cities to carry out isolation and identification of S.aureus,the drug resistance and relevant resistant and virulent genes of the isolates were detected by drug sensitive test and PCR,respectively.The results showed that 57 stains of S.aureus were isolated,with an isolation rate of 11.0%(57/520),including methicillin-resistant S.aureus(MRSA)with an isolation rate of 1.3%(7/520);the isolated strains were more resistant to penicillin(93.0%,53/57)and cotrimoxazole(80.7%,46/57),and the most sensitive to vancomycin,doxycycline and linezolid,but resistant to other antibiotics at different levels,with a multi-resistance rate of 45.6%(26/57).In addition to the virulence genes including seb,sec and sed,mecA resistance gene other than mecC was detected from 2 strains of MRSA isolates.In conclusion,pathogenic S.aureus was widely prevalent in dairy cattle farms in the 4 regions of Shandong Province and produced certain drug resistance,posing a potential threat to public health,and indicating a need to improve the prevention and control of mastitis in dairy cattle farms.
万方数据
维普
