摘要
为了解湘西自治州牛、山羊与蜱中的弓形虫感染情况,采用间接血凝法(IHA)对 7 个县采集的 203份牛血清与 238 份山羊血清进行抗体检测,同时采用PCR方法对采自牛体表的 92 只蜱进行弓形虫病原检测.结果显示:牛弓形虫抗体阳性率为 16.3%(95%CI:11.1%～21.4%),山羊为 18.5%(95%CI:13.5%～23.5%);7 个县均检出阳性抗体,不同地区之间牛、山羊血清弓形虫抗体阳性率差异均不显著(P＞0.05);雌性牛与雄性牛弓形虫阳性率分别为 12.8%、35.5%,差异有统计学意义(P＜0.05),雌性山羊和雄性山羊阳性率分别为19.1%、15.9%,差异无统计学意义(P＞0.05);在蜱体内未检测到弓形虫.结果表明:湖南省湘西自治州牛羊中普遍存在弓形虫感染,地区分布特征不明显,雄性牛感染较严重,因此要重视尤其是雄性牛羊的弓形虫防治.
Abstract
In order to investigate the prevalence of Toxoplasma gondii(T.gondii)in cattle,goats and ticks in Xiangxi Autonomous Prefecture,203 cattle and 238 goat serum samples collected from 7 counties were tested for antibodies by indirect hemagglutination(IHA),and 92 ticks collected from cattle body surfaces were tested for T.gondii by PCR.The results showed that the antibody positive rates of T.gondii were 16.3%(95%CI:11.1%-21.4%)and 18.5%(95%CI:13.5%-23.5%)in cattle and goats,respectively,and the antibodies were detected from samples of all the 7 counties,but the difference of antibody positive rates was not significant in cattle and goats in different regions(P＞0.05);the positive rates were 12.8%and 35.5%in female and male cattle,respectively,with statistically significant difference(P＜0.05),and 19.1%and 15.9%in female and male goats,respectively,without statistically significant difference(P＞0.05);no T.gondii was detected from ticks.In conclusion,T.gondii was widely distributed in cattle and goats in Xiangxi Autonomous Prefecture,Hunan Province,with unconspicuous characteristics of regional distribution,but seriously in male cattle,indicating a necessity to prevent T.gondii in cattle and goats,especially in male cattle.
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