Genome-wide Bioinformatics Analysis on HEV in Zang Pigs in Xizang Autonomous Region
In order to investigate the molecular characteristics of hepatitis E virus(HEV)derived from Xizang pigs,one strain of HEV type 4d(GenBank accession No.OQ981960)was selected for whole genome cloning,and its non-structural protein,capsid protein and phosphoprotein were bioinformatically analyzed by various bioinformatic tools.The results showed that the non-structural protein was unstable and hydrophilic without any transmembrane region or signal peptide,including 27 T cell antigenic epitopes and 29 B cell antigenic epitopes,and its secondary structure was dominated by α-helixes and random coils,while its tertiary structure contained zinc ion receptor,multiple methylation sites and a variety of deconjugating enzymes;the capsid protein was unstable and hydrophilic with transmembrane region and signal peptide,including 14 T cell antigenic epitopes and 29 B cell antigenic epitopes,its secondary structure was dominated by random coils and extended strands,while its tertiary structure contained putative glycosyl hydrolases,magnesium ion sites,synthetases,ligases,isomerases and others;phosphoprotein was stable and hydrophilic without any transmembrane region or signal peptide,including 7 T cell antigenic epitopes and 15 B cell antigenic epitopes,and its secondary structure was dominated by α-helixes and random coils,while its tertiary structure contained multiple transferases,polymerases,ligases,secretory system,methyltransferases,metal ion binding proteins,etc.A foundation was laid by the study for further understanding of the genome and protein functions of HEV derived from Zang pigs.
HEVbioinformatics analysisZang pig from Xizang Autonomous Region
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