Research Progress on CRISPR-Cas12a System and Its Application in Detecting Poultry Pathogen
The pathogenic microorganism is one of major factors affecting poultry health,seriously hindering poultry farming from rapidly growing,indicating a great significance to develop a rapid and efficient pathogen detection method for effective prevention and control of poultry diseases.CRISPR-Cas12a is an emerging gene-editing tool with high specificity,sensitivity and accessibility,which could greatly improve efficiency and accuracy of detection in combination with polymerase chain reaction(PCR),loop-mediated isothermal amplification(LAMP),recombinase polymerase amplification(RPA),recombinase-aided amplification(RAA)and other methods,and visualize test results using fluorescent signals,lateral chromatography strips and other techniques,playing an important role in pathogen detection.In the study,research progress on the action mechanism of CRISPR-Cas12a system and its application in poultry pathogen detection in combination with various nucleic acid amplification techniques,with a view to providing a reference for future diagnosis,prevention and control of poultry diseases,and laying a foundation for future development of a more effective detection platform.
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