Genomic Characterization of 4 Strains of Newcastle Disease Viruses Derived from Wild Birds in China
In order to identify the genomic characteristics of Newcastle disease virus(NDV)derived from wild birds in China,four strains of NDV were isolated from samples collected from wild birds in Ningxia,Hubei and Fujian in 2023 for genome sequencing and genetic evolution analysis.The results showed that three out of four isolates belonged to Class Ⅰ and one to Class Ⅱ,their genomes were 15 198 and 15 186 nt in length,respectively,and their cleavage site sequences of F protein were 112ERQERL117 and 112GKQGRL117,respectively;all the four isolates were identified as corresponding to the molecular characteristics of attenuated strains.Based on the analysis of amino acid sequences inferred from F and HN genes of the four isolates,it was found that,compared to most Class Ⅰ and ClassⅡ NDV isolates,several amino acid changes were observed in signal peptide(TM)region,fusion peptide region,heptapeptide repeat(HR)region of F protein,as well as N-terminal transmembrane region and stalk region(HR)of HN protein,respectively.Based on homology analysis with F gene of representative strains in NCBI database,it was found that two Class Ⅰ isolates and one Class Ⅱ isolate were the highest identical to the representative strain of 1.2 subtype,and one Class Ⅰ isolate was to 1.1.2 subtype.Genetic evolution analysis showed that two isolates belonged to Class Ⅰ 1.2 subtype,one to Class Ⅰ 1.1.2 subtype,and one to Class Ⅱ 1 subtype.In conclusion,Class I and Class Ⅱ attenuated strains were prevalent in wild birds in China,with certain mutations in their F and HN genes.The study laid a foundation for further identification of the biological characteristics of NDV derived from wild birds in China.
万方数据
维普
