Advances in the Diagnostic Techniques for Avian Infectious Bronchitis
As an acute and highly infectious respiratory disease,avian infectious bronchitis(IB)may affect chickens of all ages and breeds,bringing serious economic losses to poultry industry in China,and urging to develop rapid and effective diagnosis methods.With continuous development of diagnostic techniques,more sensitive,specific and simple techniques for IB have been developed,and become suitable for mass testing,including routine methods such as virus isolation and identification,enzyme-linked immunosorbent assay(ELISA),and molecular methods such as polymerase chain reaction(PCR)and loop-mediated isothermal amplification(LAMP).ELISA has good sensitivity and specificity,fluorescent quantitative PCR is capable of detecting RNA of the lowest viral load with higher sensitivity,LAMP and recombinant enzyme assisted amplification(RAA)can complete detection in a short time,and protein microarray method can detect multiple proteins at the same time.However,the above methods fail to fully meet the requirements of simplicity,accuracy,sensitivity and economic practicability,the most appropriate diagnostic method or a combination of several methods should be used based on experiment conditions,operating experiences and other requirements in practice.In the paper,the advances in etiology,pathology as well as serological and molecular diagnosis of IB were reviewed,with a view to technically supporting future prevention and control of the disease.
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