Effect of UDP-glucose Pyrophosphorylase galU Gene on the Pathogenicity of Salmonella typhimurium
Salmonella typhimurium(S.typhimurium)is an important zoonotic pathogen,resulting in major economic loss to animal industry in China in recent years.UDP-glucose pyrophosphorylase is encoded by galU gene and plays an important role in the synthesis of bacterial biofilm.In order to explore the correlation of galU gene to the pathogenicity of S.typhimurium,and finally to support researches on the pathogenic mechanism of the bacterial strain,the galU gene of S.typhimurium S584 strain was knocked out using Red/ET homologous recombination technology,and the biological characteristics and pathogenicity of the gene-deleted mutant were studied.The results showed that a galU-deleted mutant,S584△galU,was successfully constructed,and was generally consistent with its parental strain in the growth status at the stages of adaptation,logarithm and stability;the gene-deleted mutant lost its capacity of biofilm formation,reduced its adhesion to DF-1 cell by 76.51％,and its pathogenicity to BALB/c mice extremely significantly decreased(P＜0.01).In conclusion,galU gene was an important virulence factor to S.typhimurium.A theoretical basis was provided by the study for further clarifying pathogenic mechanism of S.typhimurium and guiding clinical diagnosis and treatment for the disease.
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