Evaluation on the Immune Effect of Six Animal Rabies Vaccines
In order to compare the immune effect of rabies vaccines commonly used in animal vaccine markets and novel vaccines under development,four inactivated vaccines(CVS-11,Flury,PV-BHK21 and SAD strain)commonly used as well as the oral vaccine(LBNSE)and the genetic engineering recombinant vaccine(booster SAD)being developed by faculties,were used to immunize the dogs with negative antibodies against rabies virus,respectively.Blood samples were collected at 30,180,and 365 days after immunization,and serum antibody levels were measured using ELISA kits.The results showed that the dogs failed to produce effective antibodies after being immunized with oral vaccine;for the recombinant vaccine,the antibody qualification rate was 100％on days 30 and 180,which decreased to 29％on days 365,failing to maintain the protective effect for a whole year.For the four inactivated vaccines,the antibody qualification rate was 100％at all the three time points,and the antibody concentration was extremely significantly higher than the oral vaccine and the recombinant vaccine(P＜0.01);on days 365,the mean antibody concentration of the dogs vaccinated with the SAD strain was extremely significantly higher than the CVS-11 strain and Flury strain(P＜0.01),and PV-BHK21 strain was significantly higher than Flury strain(P＜0.05).In conclusion,there was a gap in the immune effect of the oral vaccine and recombinant vaccine under development,compared to traditional inactivated vaccines;the four inactivated vaccines had good immune effect lasting for one year,but the antibody concentration decreased at late stage,requiring a timely vaccination every year.Supporting data was provided by the study for the application of rabies vaccines as well as the development and optimization of novel vaccines.
万方数据
维普
