尿液中大麻及合成大麻素MDMB-4en-PINACA、ADB-BUTINACA酶水解条件研究
Study on enzymatic hydrolysis conditions of cannabis,MDMB-4en-PINACA and ADB-BUTINACA in urine
张佳丽 1王鑫 2向平2
作者信息
- 1. 山西医科大学法医学院法医学山西省重点实验室,山西晋中 030600;司法鉴定科学研究院上海市法医学重点实验室上海市司法鉴定专业技术服务平台司法部司法鉴定重点实验室,上海 200063
- 2. 司法鉴定科学研究院上海市法医学重点实验室上海市司法鉴定专业技术服务平台司法部司法鉴定重点实验室,上海 200063
- 折叠
摘要
目的 合成大麻素类(synthetic cannabinoids,SCs)新精神活性物质进入人体后被广泛代谢,在尿液中通常很难检出合成大麻素原体,大多数以代谢物及代谢物葡糖醛酸结合形式存在,需在尿液前处理方法中断裂葡糖醛苷酸链,将葡萄糖醛酸结合物还原.本研究针对尿液中SCs的酶水解条件进行优化.方法 采用液相色谱串联质谱(LC-MS/MS)分析方法,对阳性尿液中11-去甲基-9-羧基-THC(Δ9-THC-COOH)的酶水解条件进行优化,并与碱消解方法进行比较;同时对MDMB-4en-PINACA、ADB-BUTINACA阳性尿液的酶水解条件进行了优化研究.结果 在55 ℃条件下,添加3 μL的β-d-葡萄糖醛苷酸酶溶液(＞100 000 units/mL)酶解30 min可充分水解Δ9-THC-COOH葡萄糖醛酸结合物,在75 ℃条件下,添加3 μL的β-d-葡萄糖醛苷酸酶溶液(＞100000 units/mL)孵育30 min可充分水解MDMB-4en-PINACA M和ADB-BUTINACA M葡萄糖醛酸结合物.结论 该研究可为检测尿液中合成大麻素类新精神活性物质建立快速、准确、可靠的酶水解方法提供参考.
Abstract
Objective Synthetic cannabinoids(SCs)will be widely metabolized to phase I metabolites and glucuronide metabolites after entering human body.It is usually difficult to detect the parent drug of SCs in urine.Therefore,it is necessary to undergo de-glucuronidation in the preparation of urine samples.We aimed to optimize the enzymatic hydrolysis methods for SCs detection in urine.Methods We studied enzymatic hydrolysis for the determination of 11-demethyl-9-carboxyl-THC(Δ9-THC-COOH)in THC positive urine samples by liquid chromatography-tandem mass spectrometry(LC-MS/MS),and compared it with the alkaline digestion method.Meanwhile,we studied enzymatic hydrolysis for the determination of the related metabolites in MDMB-4en-PINACA and ADB-BUTINACA positive urine samples.Results The Δ9-THC-COOH glucuronic acid conjugate could be hydrolyzed by adding 3μL β-d-glucuronidase solution(＞100 000 units/mL)for 30 min at 55℃.The MDMB-4en-PINACA M and ADB-BUTINACA M glucuronide conjugates were hydrolyzed by adding 3μL β-d-glucuronidase solution(＞100 000 units/mL)for 30 min at 75℃.Conclusion This study can provide reference for the establishment of rapid,accurate and reliable enzymatic hydrolysis methods when detecting SCs in urine.
关键词
法医学毒物分析/酶水解/合成大麻素/MDMB-4en-PINACA/ADB-BUTINACA/Δ9-THC-COOHKey words
Forensic toxicological analysis/Enzymatic hydrolysis/Synthetic cannabinoids(SCs)/MDMB-4en-PINACA/ADB-BUTINACA/Δ9-THC-COOH引用本文复制引用
基金项目
国家自然科学基金(82002000)
上海市法医学重点实验室项目(21DZ2270800)
上海市司法鉴定专业技术服务平台项目(19DZ2292700)
出版年
2024
NETL
NSTL
万方数据