MHSeqTyper47试剂盒在亲缘关系鉴定中的应用效能评估
Evaluating the application efficiency of the MHSeqTyper47 kit in kinship identification
檀莉帅 1金鑫 2冯耀森 3康克莱 3马温华 3李明广 4张驰 3赵杰 3叶健 1王乐3
作者信息
- 1. 山西医科大学法医学院,山西晋中 030600;法医遗传学公安部重点实验室,公安部鉴定中心,北京 100038
- 2. 海南省公安厅,海南海口 570311
- 3. 法医遗传学公安部重点实验室,公安部鉴定中心,北京 100038
- 4. 大连市公安局刑事科学技术研究所,辽宁大连 116000
- 折叠
摘要
目的 探讨MHSeqTyper47试剂盒在亲缘关系鉴定中的应用价值.方法 采用MHSeqTyper47试剂盒对113份亲缘关系个体DNA样本进行基因座复合扩增及文库构建,使用MiSeq FGx测序仪进行测序,测得的数据应用MHTyper数据分析软件进行基因分型,计算亲缘关系指数评估该试剂盒在亲缘关系鉴定中的应用效能并与GlobalFilerTM试剂盒进行比较.结果 MHSeqTyper47在三联体鉴定中CPI值1.43×1011～6.15× 1018,二联体鉴定中CPI值3.75× 105～1.53×1013,全同胞鉴定中CFSI值7.73× 101～2.59× 1016,三联体、二联体和全同胞关系对均与无关个体对可以完全区分.MHSeqTyper47和GlobalFilerTM试剂盒联用在二级亲缘关系中的系统效能为0.466 2.结论 MHSeqTyper47混合DNA鉴定试剂盒在一级亲缘关系鉴定中有较高的应用价值;与GlobalFilerTM试剂盒联用在二级亲缘关系鉴定中可以解决部分案例.
Abstract
Objective To investigate the application value of the MHSeqTyper47 kit in kinship identification.Methods Multiplexed amplification and library preparation were performed for DNA samples from 113 related individuals by using the MHSeqTyper47 kit.The libraries were sequenced on a MiSeq FGx sequencer,and the data were analyzed using MHTyper for microhaplotype genotyping.The kinship indexes were calculated to evaluate the application efficiency of this kit in kinship identification and compared with those of the GlobalFilerTM kit.Results For the MHSeqTyper47 kit,the CPI values in trio identification were 1.43× 1011～6.15×1018.The CPI values in duo identification were 1.02× 105～1.53× 1013.The CFSI values in full sibling identification were 7.73×101～2.59×1016.Trios,duos and full siblings could be completely distinguished from unrelated pairs.The combined efficiency of these two kits in 2nd-degree kinship identification was 0.466 2.Conclusion The application value of MHSeqTyper47 kit is relatively higher in the identification of lst-degree kinships.If jointly used with the GlobalFilerrM kit,2nd-degree kinship identification could be achieved in some cases.
关键词
法医遗传学/微单倍型/亲缘关系鉴定/MHSeqTyper47试剂盒/ITO法Key words
Forensic genetics/Microhaplotype/Kinship identification/MHSeqTyper47 kit/ITO method引用本文复制引用
基金项目
国家自然科学基金(81971797)
公安部技术研究计划重点项目(2017JSYJA05)
公安部科技强警基础工作专项(2022JC09)
出版年
2024
NETL
NSTL
万方数据