Comparison of five common domestic magnetic bead kits for extracting dna at different concentrations
Objective To compare the DNA extraction efficiency of five common domestic magnetic bead kits from Ruiboxiang,Xinhai Biological,Wei Dian,Bo Kun,and Wei Lue,and then provide a reference for how to choose these kits in practice.Methods Five domestic magnetic beads kits mentioned above were used to extract 007 standards(0.1 ng/μL)with the contents of 0.1 ng,0.2 ng,0.4 ng,0.6 ng,0.8 ng and 1 ng.Then the extracted DNA was quantified followed by STR analysis to compare the collecting efficiency and extraction quality of the five kits.Results When the DNA contents were 0.1 ng,0.2 ng and 0.4 ng,there were no significant differences in the recovery rate of the five kits,but the differences can be seen in STR typing results,while for 0.6 ng and above contents,the difference among the five kits begin to appear.Conclusion Any of the above kits can be used to extract DNA with the contents more than 0.6ng,but for few DNA contents,it is recommended to extracting and purifying by Xinhai biological kit.
Forensic medical evidenceMagnetic bead kitsDNA extractionRate of recovery
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