基于新一代测序技术进行孕早期流产组织胎儿STR分型及亲子鉴定
Fetal STR typing and paternity identification of early pregnancy aborted tissue based on next-generation sequencing technology
张瑾 1刘开会 1郝金萍 1杨雪莹 1张兴锟 2彭微 3徐小玉 1高珊 1畅晶晶 1雷波 2张梦楠 3王秋娟 3张颖1
作者信息
- 1. 公安部鉴定中心,北京 100038
- 2. 广州深晓基因科技有限公司,广东 广州 510663
- 3. 深圳华大法医科技有限公司,广东 深圳 518083
- 折叠
摘要
流产组织胎儿STR(short tandem repeats)分型检测长久以来始终是法医DNA检验面临的一大难题,对于孕早期流产组织无法通过组织形态学检验获取绒毛组织时,母体与胎儿混合STR分型结果可以为强奸致孕案件嫌疑人认定提供关键依据.本研究应用新一代测序技术,在4份强奸致孕早期流产组织中成功检出母体与疑似胎儿混合STR分型或疑似胎儿单一STR分型,与Y-STR、侧翼序列信息联合应用,为嫌疑人认定提供了更为全面可靠的遗传学依据.
Abstract
Fetal STR typing of aborted tissue has long been a major problem in forensic DNA.Especially for the first trimester abortion tissue,it is difficult to isolate the embryonic components by histomorphological means,resulting in the inability to accurately obtain the STR typing of the fetus.The mixed STR typing results of mother and fetus can provide a key basis for the identification of suspects in cases of rape-induced pregnancy.In this study,next generation sequencing was used to successfully detect mixed STR typing of mother and suspected fetus or single STR typing of suspected fetus in 4 rape-induced early pregnancy abortion tissues.Combined with Y-STR and flank sequence information,it provides a more comprehensive and reliable genetic basis for the identification of suspects.
关键词
法庭科学/流产组织/新一代测序/短串联重复序列/混合STR分型Key words
Forensic science/Aborted tissue/Next generation sequencing/Short tandem repeats/Mixed STR typing引用本文复制引用
基金项目
中央级公益类科研院所基本科研业务费专项资金项目(2023JB023)
中央级公益类科研院所基本科研业务费专项资金项目(2024JB007)
出版年
2024
NETL
NSTL
万方数据