首页|闭合性颅脑损伤模型中GFAP和VEGF的表达研究

闭合性颅脑损伤模型中GFAP和VEGF的表达研究

扫码查看
目的 探讨大鼠闭合性脑损伤后GFAP和VEGF的表达规律,并以此推断脑损伤后经过时间,进而为损伤时间推断提供参考.方法 选取雄性大鼠随机分为对照组和实验组,其中实验组按照损伤后经过时间不同又分损伤后1 h、6 h、12 h、1 d、3 d、7 d、14 d组.采用免疫组化法和ELISA法检测大鼠闭合性脑损伤后脑组织及血清中GFAP和VEGF的表达情况.结果 (1)免疫组化各实验组中,GFAP主要在星形胶质细胞胞浆中表达,伤后1 h开始明显增多、7 d内表达进行性增高,到伤后 14 d时表达下降接近对照组水平;VEGF主要在神经元胞浆中表达,伤后 1 d时明显增多,到伤后 3 d时达高峰、14 d时表达减少;(2)ELISA各实验组血清中VEGF含量升高早于脑组织中VEGF表达,伤后6 h开始明显增多,到伤后3 d时含量最多、伤后14 d时接近对照组.结论 (1)GFAP与VEGF表达呈现出时序性变化的特点,两者有望成为法医推断损伤时间的生物学标记物;(2)GFAP和VEGF有较高敏感性和特异性,对早期颅脑损伤时间推断有重要意义.
Expression study of GFAP and VEGF in a closed craniocerebral injury model
Objective To investigate the expression pattern of GFAP and VEGF after closed brain injury in rats,and to infer the time elapsed after brain injury,and then to provide reference for the inference of injury time.Methods Male rats were randomly divided into control group and experimental group,in which the experimental group was divided into 1 h,6 h,12 h,1 d,3 d,7 d and 14 d groups according to the different elapsed time after injury.Immunohistochemistry and ELISA were used to detect the expression of GFAP and VEGF in brain tissue and serum of rats after closed brain injury.Results(1)In each immunohistochemistry experimental group,GFAP was mainly expressed in the cytoplasm of astrocytes,with an obvious increase at 1 h after injury,a progressive increase of expression in 7 d,and a decrease of expression close to the level of the control group at 14 d after injury.While VEGF was mainly expressed in the cytoplasm of neurons,with an obvious increase at 1 d after injury,a peak at 3 d post-injury,and a decrease in expression at 14 d.(2)In each ELISA experimental group,the VEGF content in serum of each experimental group increased earlier than the VEGF expression in brain tissue,which began to increase significantly at 6 h after injury,and the content was the most at 3 d after injury,and was close to that of the control group at 14 d after injury.Conclusion(1)It has showed temporal changes in the expression of GFAP and VEGF,and both are expected to be biological markers for inferring the time of injury in forensic work.(2)GFAP and VEGF have high sensitivity and specificity and are important for the extrapolation of early craniocerebral injury time.

Forensic pathologyClosed brain injuryGFAPVEGF

张曙光、党志强、巩海霞、刘长海、贾富全

展开 >

内蒙古医科大学基础医学院法医学教研室,内蒙古 呼和浩特 010059

内蒙古医科大学内蒙古自治区分子病理学重点实验室,内蒙古 呼和浩特 010059

内蒙古迪安司法鉴定中心,内蒙古 呼和浩特 010010

乌兰察布市人民检察院,内蒙古 乌兰察布 012001

展开 >

法医病理学 闭合性脑损伤 GFAP VEGF

2024

中国法医学杂志
中国法医学会

中国法医学杂志

CSTPCD
影响因子:0.352
ISSN:1001-5728
年,卷(期):2024.39(6)