基于表面增强拉曼光谱技术的血液中苯二氮卓类药物快速检测方法
Rapid detection of illicit drugs in blood based on surface-enhanced Raman spectroscopy
沈靓 1诸仁祥 2陈芳 3施碧云 2钟武魁 4郭郡郡1
作者信息
- 1. 绍兴市公安局柯桥区分局,浙江 绍兴 312030
- 2. 绍兴文理学院,浙江 绍兴 312000
- 3. 司法鉴定科学研究院,上海 200063
- 4. 绍兴市公安局,浙江 绍兴 312000
- 折叠
摘要
目的 建立血液中阿普唑仑、硝基安定、替马西泮、地西泮、氯氮卓等 5 种苯二氮卓类违禁药品的表面增强拉曼光谱(SERS)快速检测方法.方法 采用乙腈作为提取剂对血液中的阿普唑仑、硝基安定、替马西泮、地西泮、氯氮卓进行提取,添加PSA和 C18 混合物进行优化,并使用SERS技术进行检测.结果 实验表明,阿普唑仑、硝基安定、替马西泮、地西泮、氯氮卓均出现了相应的拉曼特征信号峰,这些特征峰可以作为定性的判别依据.此外,这 5 种苯二氮卓类药物在血液中的检出限在 0.5～1 μg/mL之间.结论 本文应用表面增强拉曼技术,通过添加PSA和 C18 混合物进行前处理优化获得了良好的拉曼增强信号,该方法操作简单,分析速度快,适用于血液样品中药品的快速检测.
Abstract
Objective To establish a rapid surface-enhanced Raman spectroscopy(SERS)method for detection of five banned benzodiazepines in blood,including alprazolam,nitrovalam,Temazepam,diazepam and cloazepine.Methods Ethanol was used as extraction agent to extract nitrodiazepam,nitrazepam,diazepam and clonazepam from blood,a mixture of PSA and C18 was added for optimization,and SERS technology was used for detection.Results Experiments showed that nitalprazolam,nitrodiazepam,Temazepam,diazepam and chlorazepine all had corresponding Raman characteristic signal peaks,which could be used as the basis for qualitative discrimination.The detection limits of these benzodiazepines in blood were between 0.5 and 1 μg/mL.Conclusion This method is simple to operate and has a fast analysis speed.It is suitable for the rapid detection of drugs in blood samples.
关键词
SERS/阿普唑仑/硝基安定/替马西泮/地西泮/氯氮卓/快速检测Key words
SERS/Alprazolam/Nitrovalam/Temazepam/Diazepam/Chlorazepine/Quick detection引用本文复制引用
出版年
2024
NETL
NSTL
万方数据