Correlation between Peripheral Blood MiR-125b-5p Level and the Severity of Coronary Artery Calcification in Maintenance Hemodialysis Patients
Objective To analyze the relationship between peripheral blood miR-125b-5p level and the severity of coronary artery calcification(CAC)in maintenance hemodialysis(MHD)patients,and investigated possible mechanism.Methods Patients who underwent maintenance hemodialysis from January 2021 to May 2023 were selected.All participants underwent chest cardiac CT examination and assayed peripheral blood miR-125b-5p levels.Exploring the effects of high phosphorus and miR-125b-5p expression on cellular calcium deposition and inflammatory protein expression in mouse thoracic aortic smooth muscle cells(SMCs).Results A total of 117 patients were included in the study,with 69 males(59％)and 48 females(41％),with an average age of 62 years.According to the CAC score,there were 71 patients in the mild CAC(＜400 points)group and 46 patients in the severe CAC(≥400 points)group.Multivariate logistic regression analysis showed peripheral blood miR-125b-5p levels(OR=0.325,95％CI:0.205-0.517,P＜0.001)were independently correlated with the severity of CAC.Receiver operator characteristic(ROC)curve showed miR-125b-5p predicted severe CAC with the area under the curve(AUC)of 0.831(95％CI:0.750-0.894,P＜0.001).The cell experiment results showed that compared to the control group,high phosphorus intervention significantly increased calcium deposition in SMCs,and TLR4 and NF-κB P65 protein were upregulated simultaneously(P＜0.05).Compared to the high phosphorus group,overexpression of miR-125b-5p significantly reduced high phosphorus induced calcium deposition and alleviatedproteins of TLR4 and NF-κB p65 expression upregulation(P＜0.05).Conclusion Peripheral blood miR-125b-5p is significantly negatively correlated with the severity of CAC in MHD patients,the mechanism may be reduce vascular calcification by reducing inflammatory response.
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