Chronic Stress Promotes the Progression of Carotid Atherosclerosis through Focal Adhesion Pathway:Evidence from Mouse Models
Objective To verify the possible mechanism of chronic stress in the occurrence and development of carotid atherosclerosis.Methods C57BL/6J and Apoe-/-mice were divided into normal control,chronic stress,atherosclerosis and atherosclerosis+chronic stress groups.Study the role of focal adhesion pathway through protein blotting experiments.Finally,bioinformatics methods were used to validate pathways in public databases.Results The chronic stress(CS)mice model and atherosclerosis(AS)mice model were constructed and evaluated.Western blotting showed that the expression of Focal adhesion pathway protein in AS mice was reduced,and that in CS+AS mice,the expression of Focal adhesion pathway protein was further reduced,accompanied by increased inflammatory factors.Identify differentially expressed genes using bioinformatics methods and enrich them into the Focal adhesion pathway.Protein interaction network(PPI)analysis showed that the core genes FLNA and MYH9 in this pathway were associated with carotid atherosclerosis.Conclusion In chronic stress,focal adhesion pathway plays a key role in promoting the development of atherosclerosis.MYH9 and FLNA have been identified as potential key genes in this process.
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