Relationship between Peripheral Blood miR-23,NF-κB,TGF-β1 and Cardiac Function Grading in Patients with Rheumatic Heart Disease
Objective To explore the relationship between peripheral blood microRNA-23(miR-23),nuclear factor kappa B(NF-κB),transforming growth factor-β1(TGF-β1)and cardiac function grading in patients with rheumatic heart disease(RHD).Methods A total of 74 patients with RHD and 74 healthy controls were enrolled as RHD group and control group between January 2019 and January 2022.The levels of TGF-β1 and NF-κB in peripheral blood were detected by enzyme-linked immunosorbent assay,expression of peripheral blood miR-23 was detected by fluorescence quantitative PCR,and cardiac function indexes were examined by echocardiography.The relationship between peripheral blood miR-23,NF-κB,TGF-β1 and grading of New York Heart Association(NYHA)was analyzed by Spearman correlation analysis.Results The levels of peripheral blood miR-23 and NF-κB in RHD group were significantly higher than those in control group,while LVEF and TGF-β1 were significantly lower than those in control group(all P＜0.05).There were significant differences in expression levels of miR-23,NF-κB and TGF-β1 among RHD patients with different NYHA grading(all P＜0.05).The peripheral blood miR-23 and NF-κB were negatively correlated with NYHA grading,while TGF-β1 was positively correlated with NYHA grading(all P＜0.05).Conclusion The level of peripheral blood NF-κB is increased,while TGF-β1 level is decreased in patients with RHD,and the two indexes are closely related to cardiac function grading.
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