Preliminary Screening of the Key Genes of Vasculopathy in Hypertensive Intracerebral Hemorrhage
Objective To screen out the key genes of hypertensive intracerebral hemorrhage(HICH)and provide new candidate targets for the preclinical intervention of HICH.Methods The Mouse Genome Informatics(MGI)intracerebral hemorrhage phenotype gene set(MGI-149)was established by using the MGI database.The cerebrovascular differential expression gene set of hypertensive intracerebral hemorrhage(HICH-DEG)was established by building the HICH animal models and conducting an analysis of cerebrovascular transcriptome sequencing.The gene ontology(GO)/protein-protein interaction(PPI)scoring system was constructed through GO enrichment analysis and PPI analysis.The GIC scoring system was also constructed by gene importance analysis.We used both scoring systems to screen out the key genes from the overlapping genes of the two gene sets and the HICH-DEG gene set respectively.GeneMANIA was employed to analyze and predict the underlying interaction targets of these primary screened genes,followed by enrichment analysis of them to understand which potential biological functions or pathways may be involved in the pathogenesis of HICH vasculopathy.Moreover,we searched articles related to the screened genes to inspect and verify their relationships with HICH.Results Through GeneMANIA prediction,enrichment analysis based on prediction,and literature research,we discarded genes Zfp697 and Gm11738 that lacked prediction results and literature support,and ultimately obtained five genes,Ctnnb1,Kdr,Syk,Tnf,and Abca1,as the key genes for initial screening.Conclusion Genes Ctnnb1,Kdr,Syk,Tnf,and Abca1 are screened as key genes.They may play important roles in the vascular pathogenesis of HICH and become important targets of preclinical intervention.
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