Lonicera Flavone Inhibits Ox-LDL-induced Proliferation and Migration of Vascular Smooth Muscle Cells through Regulating miR-134-5p
Objective To investigate the effect and possible mechanism of lonicera flavonoids on the proliferation and migration of oxidized low-density lipoprotein(Ox-LDL)-induced vascular smooth muscle cells(VSMCs).Methods Effects of lonicera flavonoids(62.5,125,250 μg/mL)on the proliferation and migration of Ox-LDL-induced VSMCs were calculated by MTT experiment,scratch healing experiment,and Transwell experiment.Western blotting was used to detect E-cadherin and N-cadherin expression.RT-qPCR was employed to detect miR-134-5p expression.The influence of miR-134-5p inhibition or miR-134-5p overexpression combined with lonicera flavonoids on the proliferation and migration of Ox-LDL-induced VSMCs were assessed using the above methods.Results The activity,migration distance,migration number,N-cadherin and miR-134-5p of VSMCs were significantly increased after Ox-LDL treatment,while E-cadherin was significantly decreased.The treatment of lonicera flavonoids significantly reduced the activity,migration distance,migration number,and N-cadherin expression as well as miR-134-5p expression of Ox-LDL-induced VSMCs,while increased the expression of E-cadherin.miR-134-5p inhibition significantly reduced the activity,migration distance,migration number and N-cadherin protein expression of Ox-LDL-induced VSMCs,while increased E-cadherin expression.miR-134-5p overexpression significantly weakened the effects of lonicera flavonoids treatment on the activity and migration,as well as N-cadherin and E-cadherin expression of Ox-LDL-induced VSMCs.Conclusion Lonicera flavonoids may inhibit the proliferation and migration of Ox-LDL-induced VSMCs by down-regulating miR-134-5p.
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