Determination of 52 antibiotics in water through a fully automated solid-phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry system
Objective To develop a fully automated method combining solid-phase extraction with ultra-high performance liquid chromatography tandem mass spectrometry for the determination of 52 types of antibiotics in drinking water.Methods The water samples were adjusted to pH 2 using phosphoric acid.The antibiotics in the water samples were enriched and purified through solid-phase extraction membrane.Subsequently,the samples underwent two elutions with 10 mL of 5％ammonia methanol,followed by nitrogen-assisted drying to near-dryness.Finally,the volume was adjusted to 1 mL using a 5％methanol aqueous solution for analysis purposes.Separation was achieved using an ACQUITY UPLC BEH C18 chromatographic column(2.1x100 mm,1.7 μm).A mobile phase consisting of a gradient elution system comprising of 0.1％formic acid-water-0.1％formic acid methanol was employed for this purpose.Mass spectrometry utilized positive ion scan multiple reaction monitoring(MRM)mode.Results Within the range of 0.1-100 μg/L,all regression equations obtained for the 52 target analytes exhibited satisfactory linear relationships.The method detection limit ranged from 0.1 to 0.6 ng/L,while the recovery rate varied between 65.1％and 133.3％.Additionally,the relative standard deviation(RSD)ranged from 3.1％to 20.5％.Conclusion The established method is a simple,rapid,and highly accurate and precise approach for the simultaneous detection of 52 types of antibiotics in drinking water.
solid-phase extractionultra-high performance liquid chromatography-tandem mass spectrometryantibiotics
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