首页|2019-2022年宁夏回族自治区炭疽时空聚集性及炭疽芽孢杆菌分子分型分析

2019-2022年宁夏回族自治区炭疽时空聚集性及炭疽芽孢杆菌分子分型分析

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
目的 对2019-2022年宁夏回族自治区炭疽病例进行时空聚集性分析和同时期分离的14株炭疽芽孢杆菌进行分子分型分析.方法 收集宁夏回族自治区2019-2022年发生的炭疽病例资料,以区县为单位应用SaTScan软件进行时空聚类分析;对2019-2021年分离的全部14株炭疽芽孢杆菌采用CanSNPs、MLVA-25、SNR-4方法进行分子分型分析.结果 2019-2022年宁夏回族自治区共报告炭疽病80例,均为皮肤炭疽病例.SaTScan时空聚类分析显示,2021年1月1日—2022年12月31日在宁夏中北部存在时空聚集区,其中永宁县存在一类聚集区(P＜0.01,RR=13.83),同期在灵武市和吴忠市利通区存在二类聚集区(P=0.022,RR=3.47).同时期分离的14株炭疽芽孢杆菌的遗传关系被定义为:A.Br001/002亚群、A3.b基因簇内6种不同的MLVA基因型,其中存在3个共享基因型,并分别被SNR-4分析定义为2、3、5种PHRANA基因型;SNR-4分析显示有12种SNR基因型.结论 2019-2022年宁夏回族自治区炭疽病发病率呈上升趋势.2021-2022年形成炭疽病的时空聚集区的原因之一可能是自然疫源地形成和发展.通过分子分型发现,2019-2022年宁夏回族自治区分离出的菌株遗传关系较为单一,同一遗传关系下遗传多样性丰富.
Spatiotemporal clustering of anthrax and molecular typing of Bacillus anthracis in Ningxia Hui Autonomous Region,2019-2022
Objective To analyze the spatiotemporal clustering of anthrax cases in the Ningxia Hui Autonomous Region from 2019 to 2022 and to perform molecular typing of 14 strains of Bacillus anthracis(B.anthracis)isolated during the same period.Methods Data on anthrax cases reported in Ningxia Hui Autonomous Region(Ningxia)from 2019 to 2022 were collected,and spatiotemporal cluster analysis was performed using SaTScan software with districts and counties as units.A total of 14 strains of B.anthracis isolated from local anthrax cases from 2019 to 2021 were subjected to molecular typing analysis using canonical single nucleotide polymorphisms(CanSNPs),twenty-five marker multilocus variable number tandem repeat analysis(MLVA-25),and single nucleotide repeat(SNR-4)methods.Results A total of 80 anthrax cases,all of which were cutaneous anthrax,were reported in Ningxia during the 3-year period.SaTScan spatiotemporal cluster analysis revealed a spatiotemporal cluster in north-central Ningxia from 2021 to 2022,with a primary cluster in Yongning county[P＜0.01,relative risk(RR)=13.83]and secondary clusters in Lingwu city and Litong district(P=0.022,RR=3.47).The genetic relationships of the 14 B.anthracis strains isolated during the same period were defined as:A.Br 001/002 subgroup,6 different MLVA genotypes within A3.b gene cluster,of which 3 common genotypes were defined as 2,3,and 5 PHANA genotypes,respectively,by SNR-4 analysis;SNR-4 analysis showed 12 SNR genotypes.Conclusion The incidence of anthrax in Ningxia showed an increasing trend from 2019 to 2022.One of the reasons for the formation of spatiotemporal clusters of anthrax in 2021-2022 may be the formation and development of natural foci.Molecular typing revealed that the genetic relationship of the strains isolated in Ningxia from 2019 to 2022 was relatively homogeneous,with abundant genetic diversity within the same genetic relationship.

anthraxtwenty-five marker multi-locus variable number tandem repeat analysiscanonical single nucleotide polymorphismssingle nucleotide repeatspatiotemporal cluster analysisNingxia Hui Autonomous Region

冼然、杨聪、高建炜、高磊、陈倩、赵瑜、蒯文和、樊浩浩、李筱笛、马江涛

展开 >

宁夏回族自治区疾病预防控制中心,银川 750004

宁夏医科大学公共卫生学院流行病与卫生统计学系,银川 750001

炭疽 MLVA-25 CanSNPs SNR-4 时空聚类分析 宁夏回族自治区

宁夏自然科学基金宁夏自然科学基金

2022AAC037122023AAC02075

2024

10.11847/zgggws1144025

中国公共卫生
中华预防医学会

中国公共卫生

CSTPCD北大核心
影响因子：1.404
ISSN：1001-0580
年,卷(期)：2024.40(10)