Objective To Analyzing the infection status and epidemiological characteristics of mycoplasma pneumoniae(MP)in children,and provide a theoretical basis for the prevention and control of MP infections among children.Methods Collecting medical records data from hospitalized children at Lanzhou Maternity and Child Health Care Hospital from 2018 to 2021.Using children hospitalized in 2018-2019 as the control group and those hospitalized in 2020-2021 as the observation group.Performing statistical analysis by using SPSS 22.0 software.Results The positive rate of MP-IgM antibodies was significantly higher in the observation group than in the control group among females compared to males,with statistical significance(x2=13.687,5.077,P<0.05);the detection rate of MP-IgM antibodies was highest among pneumonia patients,the difference was statistically significant compared with children with other diseases(P<0.01);the detection rate of MP-IgM antibodies in the observation group of patients was significantly lower than that in the control group,with statistical significance(x2=37.162,P<0.05).In the control group,children showed a relatively higher detection rate of MP-IgM antibodies during the winter season,with statistical significance(x2=28.705,P<0.05);however,in the observation group,there was no significant peak in MP-IgM antibody detection rate throughout the year.Conclusion During the COVID-19 pandemic,measures such as frequent handwashing,proper mask-wearing,and working from home effectively reduced the infection rate of MP among children in Lanzhou.It is recommended to strengthen infectious disease prevention and control in the future,implement targeted non-pharmaceutical interventions to prevent outbreaks and epidemics of infectious diseases.