Investigation on Bartonella carried by rodents at Anhui ports
Objective To investigate the natural infection of Bartonella in rodents at Anhui ports.Methods From 2019 to 2021,the rodents were captured by traps at 7 ports of Anhui.DNA was extracted from spleens samples and Bartonella gh A gene was detected by PCR.The PCR positive products were sequenced,the sequences were compared and analyzed via Mega11.Results A total of 24 rodents only from 3 Apodemus agrarius,Rattus tanezumi,Rattus norvegi-cus,Mus muscuhus,Rattus nitidus,Niviventer confucianus and Crocidura lasiura were captured at 7 ports of Anhui.Apodemus agrarius accounted for 29.17％of the total.The positive rate of nucleic acid detection of Bartonella was 12.50％(3/24)in the spleens only from 3 Apodemus agrarius.The sequencing results showed that BT438 and BT453 were the same as Uncultured Bartonella strain,and BT473 was the same as Bartonella fuyuanensis strain.Conclusion Natural Bartonella infection existed in rodents at Anhui ports,and there was diversity of Bartonella species.It should be strengthened the surveillance of pathogens carried by rodents and rodent ectoparasites.
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