Surrey on norovirus contamination in different water in Guiyang
Objective To understand the contamination status of different water by norovirus in Guiyang City,and to provide data reference for water source management and norovirus epidemic prevention and control.Methods In December 2022,a total of 336 water samples including source water,river water,tap water and barreled water were collected from 10 districts and counties in Guiyang.After being enriched by using Concentration and Purification System(CPS)for microorganisms,RNA of norovirus was detected by Real-time PCR,the positive samples were typed for GⅠ and GⅡ.Results No norovirus was detected positive in terminal water and barreled water before and after enrichment.The positive rate of norovirus in river water before enrichment was 50.00％(20/40),the positive rate of source water was 6.25％(3/48).After enrichment,the positive rate of river water increased to 90.00％(36/40),and the positive rate of source water increased to 25.00％(12/48).There was statistical significance in the positive rates of river water and source water before and after enrichment(x2=13.393,P＜0.00 1;x2=5.057,P＜0.05).The positive rates of GⅠ,GⅡ and GⅠ+GⅡ were 2.27％(4/176),12.50％(22/176)and 25.57％(45/176),respectively.Conclusion Norovirus contamination existed in source water and river water in Guiyang City,with GⅠ+GⅡ types contamination were the main contaminants,followed by GⅡ.
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